Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Editionpresents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosisin vitroandin vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane and organelle trafficking in cultured cells and powerfulin vivoimaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Highly practical and clearly written, Exocytosis and Endocytosis, Second Editionwill serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms. | Exocytosis and Endocytosis by Andrei I. Ivanov, Hardcover | Indigo Chapters